The Prevalence of Colonization with Carbapenem-resistant Enterobacteriaceae, E. coli, Klebsiella and Enterobacter, and Related Risk Factors in Children

Background: Carbapenems are broad-spectrum antibiotics used to treat the family of gram-negative Enterobacteriaceae, especially those that resistant first-line antibiotics. Because these drugs usually prescribed as last line treatment, resistance carries irreparable risks treatment systems, and screening high-risk individuals in medical centers using infection control measures critical strategies for eliminating them. Objectives: We investigated prevalence colonization different strains Klebsiella, Enterobacter, Escherichia coli their risk factors hospitalized children. Methods: In this descriptive cross-sectional study, stool samples were taken from patients during first 48 hours hospitalization a tertiary children’s hospital cultured on Makcanki culture medium or EMB. Cultured Enterobacteriaceae transferred Müller-Hinton agar medium, antibiotic susceptibility was evaluated with meropenem imipenem discs by disc diffusion method. next step, five common carbapenemase genes, including (VIM, IMP, OXA-48, NDM-1, SPM-1) examined PCR method reported accordingly. Results: Two hundred ninety-five examined, which 242 (82%) positively Enterobacteriaceae. The carbapenem be 37% among 295 phenotypic Resistance rates high history use, frequent hospitalizations (more than two episodes six months), an underlying disease) malignancy, GI diseases, immunodeficiency, neurologic diseases such cerebral palsy epilepsy, endocrine diseases. Most genes found followed IMP VIM. NDM-1 3 samples, SPM not any samples. 13% more one gene found. Conclusions: results study showed frequency stools colonized is our patients. On other hand, presence bacteria, located plasmids can rapidly spread environment, alarm committee take preventive order prevent bacteria hospital.